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Genomics Master Thesis

Author: Siva Subramanian Ayeraselvan
Date: June 2025


Project Description

This repository contains the scripts, data files, and documentation associated with my Master's thesis in Genomics. The project focuses on analyzing genomic datasets to address biological questions related to:

  • Adaptation
  • Niche modeling
  • Conservation genomics

The analyses combine next-generation sequencing (NGS) data processing with advanced statistical and ecological modeling.


Repository Structure

├── NGS_genomic_analysis 
├── GEMMA/ # GWAS / GEA genome environmental association scripts
│ └── GEA_scripts/ 
├── mar/ # mutation area relationship - conservation genomics
├── maxent_manual/ # detailed overview to perform maxent
├── gradient_forest/ # GF analysis i performed
└── README.md # This file

Short overview

The following methods and analyses are included:

  • Genome-Environment Associations using GEMMA
  • Species Distribution Modeling with MaxEnt
  • Gradient Forest analysis for adaptive genomic variation
  • Mutation-Area Relationship (MAR) analysis for conservation applications

Software and Tools

  • Python >= 3.8
  • R >= 4.2
  • GEMMA
  • maxENT
  • Gradient Forest (R package)
  • BEDTools
  • VCFtools
  • Samtools
  • BCFtools

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NGS genomics, Niche modelling, and Extinction scenario simulation - MAR prediction curve

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