Nanopore is a cost-effective technology for rapid sequencing, but it is somewhat error prone. Can it be used to quantify enrichment of Nanobodies in selection experiments?
Consider an experiment where there are two rounds of enrichment with libraries
- barcode01 => unpanned
- barcode02 => panned once
- barcode03 => panned twice
We first cluster the sequences using MMSeqs2
Then for quantify the enrichment for the two stages:
