diff --git a/CHANGELOG.md b/CHANGELOG.md index 0b355fb..b4792bd 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,7 @@ ## 1.2.0 - Fix: Handle BAM records with empty SEQ fields (`*` in SAM format) in `base-depth`. Previously this would cause an out-of-bounds error. Now these reads still count toward depth, and their bases are counted as `N`. ([#92](https://github.com/sstadick/perbase/pull/92) by @ghuls) +- Chore: Update deps and fixup lints ## 1.1.0 diff --git a/Cargo.lock b/Cargo.lock index 9f1f1a2..29481da 100644 --- a/Cargo.lock +++ b/Cargo.lock @@ -3,16 +3,16 @@ version = 4 [[package]] -name = "adler" -version = "1.0.2" +name = "adler2" +version = "2.0.1" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "f26201604c87b1e01bd3d98f8d5d9a8fcbb815e8cedb41ffccbeb4bf593a35fe" +checksum = "320119579fcad9c21884f5c4861d16174d0e06250625266f50fe6898340abefa" [[package]] name = "aho-corasick" -version = "1.1.2" +version = "1.1.4" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "b2969dcb958b36655471fc61f7e416fa76033bdd4bfed0678d8fee1e2d07a1f0" +checksum = "ddd31a130427c27518df266943a5308ed92d4b226cc639f5a8f1002816174301" dependencies = [ "memchr", ] @@ -23,14 +23,64 @@ version = "0.12.1" source = "registry+https://github.com/rust-lang/crates.io-index" checksum = "d52a9bb7ec0cf484c551830a7ce27bd20d67eac647e1befb56b0be4ee39a55d2" dependencies = [ - "winapi 0.3.9", + "winapi", +] + +[[package]] +name = "anstream" +version = "0.6.21" +source = "registry+https://github.com/rust-lang/crates.io-index" +checksum = "43d5b281e737544384e969a5ccad3f1cdd24b48086a0fc1b2a5262a26b8f4f4a" +dependencies = [ + "anstyle", + "anstyle-parse", + "anstyle-query", + "anstyle-wincon", + "colorchoice", + "is_terminal_polyfill", + "utf8parse", +] + +[[package]] +name = "anstyle" +version = "1.0.13" +source = "registry+https://github.com/rust-lang/crates.io-index" +checksum = "5192cca8006f1fd4f7237516f40fa183bb07f8fbdfedaa0036de5ea9b0b45e78" + +[[package]] +name = "anstyle-parse" +version = "0.2.7" +source = "registry+https://github.com/rust-lang/crates.io-index" +checksum = "4e7644824f0aa2c7b9384579234ef10eb7efb6a0deb83f9630a49594dd9c15c2" +dependencies = [ + "utf8parse", +] + +[[package]] +name = "anstyle-query" +version = "1.1.5" +source = "registry+https://github.com/rust-lang/crates.io-index" +checksum = "40c48f72fd53cd289104fc64099abca73db4166ad86ea0b4341abe65af83dadc" +dependencies = [ + "windows-sys", +] + +[[package]] +name = "anstyle-wincon" +version = "3.0.11" +source = "registry+https://github.com/rust-lang/crates.io-index" +checksum = "291e6a250ff86cd4a820112fb8898808a366d8f9f58ce16d1f538353ad55747d" +dependencies = [ + "anstyle", + "once_cell_polyfill", + "windows-sys", ] [[package]] name = "anyhow" -version = "1.0.75" +version = "1.0.100" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "a4668cab20f66d8d020e1fbc0ebe47217433c1b6c8f2040faf858554e394ace6" +checksum = "a23eb6b1614318a8071c9b2521f36b424b2c83db5eb3a0fead4a6c0809af6e61" [[package]] name = "approx" @@ -49,20 +99,40 @@ checksum = "d9b39be18770d11421cdb1b9947a45dd3f37e93092cbf377614828a319d5fee8" dependencies = [ "hermit-abi 0.1.19", "libc", - "winapi 0.3.9", + "winapi", ] [[package]] name = "autocfg" -version = "1.1.0" +version = "1.5.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "d468802bab17cbc0cc575e9b053f41e72aa36bfa6b7f55e3529ffa43161b97fa" +checksum = "c08606f8c3cbf4ce6ec8e28fb0014a2c086708fe954eaa885384a6165172e7e8" + +[[package]] +name = "bindgen" +version = "0.69.5" +source = "registry+https://github.com/rust-lang/crates.io-index" +checksum = "271383c67ccabffb7381723dea0672a673f292304fcb45c01cc648c7a8d58088" +dependencies = [ + "bitflags 2.10.0", + "cexpr", + "clang-sys", + "itertools 0.12.1", + "lazy_static", + "lazycell", + "proc-macro2 1.0.103", + "quote 1.0.42", + "regex", + "rustc-hash", + "shlex", + "syn 2.0.111", +] [[package]] name = "bio" -version = "0.41.0" +version = "3.0.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "4a69423e30444738eccc5e54eccee75779dd3f15ecc0469b95d8529d4b6b7586" +checksum = "2169c40f17068bcf0563e5060ed6ccab694f68068133055f6e96bcdf987b3f32" dependencies = [ "anyhow", "approx", @@ -72,12 +142,13 @@ dependencies = [ "bytecount", "csv", "custom_derive", + "derefable", + "editdistancek", "enum-map", "fxhash", "getset", - "itertools", + "itertools 0.12.1", "itertools-num", - "lazy_static", "multimap", "ndarray", "newtype_derive", @@ -85,45 +156,44 @@ dependencies = [ "num-traits", "ordered-float", "petgraph", - "rand", "regex", "serde", "serde_derive", "statrs", "strum 0.23.0", - "strum_macros 0.23.1", - "thiserror", + "strum_macros 0.26.4", + "thiserror 2.0.17", "triple_accel", "vec_map", ] [[package]] name = "bio-types" -version = "1.0.1" +version = "1.0.4" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "9d45749b87f21808051025e9bf714d14ff4627f9d8ca967eade6946ea769aa4a" +checksum = "f4dcf54f8b7f51450207d54780bab09c05f30b8b0caa991545082842e466ad7e" dependencies = [ - "derive-new", + "derive-new 0.6.0", "lazy_static", "regex", - "strum_macros 0.25.2", - "thiserror", + "strum_macros 0.26.4", + "thiserror 1.0.69", ] [[package]] name = "bit-set" -version = "0.5.3" +version = "0.8.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "0700ddab506f33b20a03b13996eccd309a48e5ff77d0d95926aa0210fb4e95f1" +checksum = "08807e080ed7f9d5433fa9b275196cfc35414f66a0c79d864dc51a0d825231a3" dependencies = [ "bit-vec", ] [[package]] name = "bit-vec" -version = "0.6.3" +version = "0.8.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "349f9b6a179ed607305526ca489b34ad0a41aed5f7980fa90eb03160b69598fb" +checksum = "5e764a1d40d510daf35e07be9eb06e75770908c27d411ee6c92109c9840eaaf7" [[package]] name = "bitflags" @@ -133,15 +203,15 @@ checksum = "bef38d45163c2f1dde094a7dfd33ccf595c92905c8f8f4fdc18d06fb1037718a" [[package]] name = "bitflags" -version = "2.4.0" +version = "2.10.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "b4682ae6287fcf752ecaabbfcc7b6f9b72aa33933dc23a554d853aea8eea8635" +checksum = "812e12b5285cc515a9c72a5c1d3b6d46a19dac5acfef5265968c166106e31dd3" [[package]] name = "bstr" -version = "1.7.0" +version = "1.12.1" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "c79ad7fb2dd38f3dabd76b09c6a5a20c038fc0213ef1e9afd30eb777f120f019" +checksum = "63044e1ae8e69f3b5a92c736ca6269b8d12fa7efe39bf34ddb06d102cf0e2cab" dependencies = [ "memchr", "regex-automata", @@ -150,9 +220,9 @@ dependencies = [ [[package]] name = "bumpalo" -version = "3.14.0" +version = "3.19.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "7f30e7476521f6f8af1a1c4c0b8cc94f0bee37d91763d0ca2665f299b6cd8aec" +checksum = "46c5e41b57b8bba42a04676d81cb89e9ee8e859a1a66f80a5a72e1cb76b34d43" [[package]] name = "bv" @@ -166,9 +236,9 @@ dependencies = [ [[package]] name = "bytecount" -version = "0.6.4" +version = "0.6.9" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "ad152d03a2c813c80bb94fedbf3a3f02b28f793e39e7c214c8a0bcc196343de7" +checksum = "175812e0be2bccb6abe50bb8d566126198344f707e304f45c648fd8f2cc0365e" [[package]] name = "byteorder" @@ -178,36 +248,47 @@ checksum = "1fd0f2584146f6f2ef48085050886acf353beff7305ebd1ae69500e27c67f64b" [[package]] name = "bytes" -version = "1.5.0" +version = "1.11.0" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "a2bd12c1caf447e69cd4528f47f94d203fd2582878ecb9e9465484c4148a8223" +checksum = "b35204fbdc0b3f4446b89fc1ac2cf84a8a68971995d0bf2e925ec7cd960f9cb3" [[package]] -name = "bzip2-sys" -version = "0.1.11+1.0.8" +name = "cc" +version = "1.2.49" source = "registry+https://github.com/rust-lang/crates.io-index" -checksum = "736a955f3fa7875102d57c82b8cac37ec45224a07fd32d58f9f7a186b6cd4cdc" +checksum = "90583009037521a116abf44494efecd645ba48b6622457080f080b85544e2215" dependencies = [ - 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pileup.set_max_depth(std::cmp::min( - i32::max_value().try_into().unwrap(), - self.max_depth, - )); + pileup.set_max_depth(std::cmp::min(i32::MAX.try_into().unwrap(), self.max_depth)); let result: Vec = pileup .flat_map(|p| { let pileup = p.expect("Extracted a pileup"); diff --git a/src/commands/merge_adjacent.rs b/src/commands/merge_adjacent.rs index d7af29b..0278305 100644 --- a/src/commands/merge_adjacent.rs +++ b/src/commands/merge_adjacent.rs @@ -71,7 +71,7 @@ impl MergeAdjacent { }; let mut curr_depth = first.depth; - while let Some(bedlike) = iter.next() { + for bedlike in iter { if bedlike.depth == curr_depth && curr_end >= bedlike.pos && bedlike.ref_seq == curr_seq diff --git a/src/commands/only_depth.rs b/src/commands/only_depth.rs index 42ee15d..04d284d 100644 --- a/src/commands/only_depth.rs +++ b/src/commands/only_depth.rs @@ -147,7 +147,7 @@ impl OnlyDepth { self.bcf_file.clone(), !self.skip_merging_intervals, Some(cpus), - Some(self.chunksize.clone()), + Some(self.chunksize), Some(self.channel_size_modifier), processor, ); @@ -205,6 +205,7 @@ struct OnlyDepthProcessor { impl OnlyDepthProcessor { /// Create a new OnlyDepthProcessor + #[allow(clippy::too_many_arguments)] fn new( reads: PathBuf, ref_fasta: Option, @@ -307,7 +308,7 @@ impl OnlyDepthProcessor { for record in reader .rc_records() .map(|r| r.expect("Read record")) - .filter(|read| self.read_filter.filter_read(&read, None)) + .filter(|read| self.read_filter.filter_read(read, None)) .flat_map(|record| IterAlignedBlocks::new(record, self.mate_fix)) { let rec_start = u32::try_from(record.0).expect("check overflow"); @@ -396,7 +397,7 @@ impl OnlyDepthProcessor { for record in reader .rc_records() .map(|r| r.expect("Read record")) - .filter(|read| self.read_filter.filter_read(&read, None)) + .filter(|read| self.read_filter.filter_read(read, None)) { let rec_start = u32::try_from(record.reference_start()).expect("check overflow"); let rec_stop = u32::try_from(record.reference_end()).expect("check overflow"); diff --git a/src/lib/par_granges.rs b/src/lib/par_granges.rs index a767abe..613ca3c 100644 --- a/src/lib/par_granges.rs +++ b/src/lib/par_granges.rs @@ -87,10 +87,11 @@ impl ParGranges { /// * `merge_regions` - If `regions_bed` and or `regions_bcf` is specified, and this is true, merge any overlapping regions to avoid duplicate output. /// * `threads`- Optional threads to restrict the number of threads this process will use, defaults to all /// * `chunksize`- optional argument to change the default chunksize of 1_000_000. `chunksize` determines the number of bases - /// each worker will get to work on at one time. + /// each worker will get to work on at one time. /// * `channel_size_modifier`- Optional argument to modify the default size ration of the channel that `R::P` is sent on. - /// formula is: ((BYTES_INA_GIGABYTE * channel_size_modifier) * threads) / size_of(R::P) + /// formula is: ((BYTES_INA_GIGABYTE * channel_size_modifier) * threads) / size_of(R::P) /// * `processor`- Something that implements [`RegionProcessor`](RegionProcessor) + #[allow(clippy::too_many_arguments)] pub fn new( reads: PathBuf, ref_fasta: Option, @@ -109,7 +110,7 @@ impl ParGranges { }; // Keep two around for main thread and thread running the pool - let threads = std::cmp::max(threads.checked_sub(2).unwrap_or(0), 1); + let threads = std::cmp::max(threads.saturating_sub(2), 1); let pool = rayon::ThreadPoolBuilder::new() .num_threads(threads) .build() @@ -198,10 +199,7 @@ impl ParGranges { }; // The number positions to try to process in one batch - let serial_step_size = self - .chunksize - .checked_mul(self.threads as u32) - .unwrap_or(u32::MAX); // aka superchunk + let serial_step_size = self.chunksize.saturating_mul(self.threads as u32); // aka superchunk for (tid, intervals) in intervals.into_iter().enumerate() { let tid: u32 = tid as u32; let tid_end: u32 = header.target_len(tid).unwrap().try_into().unwrap(); @@ -210,8 +208,7 @@ impl ParGranges { let mut result = vec![]; for chunk_start in (0..tid_end).step_by(serial_step_size as usize) { let tid_name = std::str::from_utf8(header.tid2name(tid)).unwrap(); - let chunk_end = - std::cmp::min(chunk_start as u32 + serial_step_size, tid_end); + let chunk_end = std::cmp::min(chunk_start + serial_step_size, tid_end); trace!( "Batch Processing {}:{}-{}", tid_name, chunk_start, chunk_end @@ -259,15 +256,15 @@ impl ParGranges { for tid in 0..(header.target_count()) { let tid_len: u32 = header.target_len(tid).unwrap().try_into().unwrap(); for start in (0..tid_len).step_by(chunksize as usize) { - let stop = std::cmp::min(start as u32 + chunksize, tid_len); + let stop = std::cmp::min(start + chunksize, tid_len); intervals[tid as usize].push(Interval { - start: start as u32, - stop: stop, + start, + stop, val: (), }); } } - Ok(intervals.into_iter().map(|ivs| Lapper::new(ivs)).collect()) + Ok(intervals.into_iter().map(Lapper::new).collect()) } /// Read a bed file into a vector of lappers with the index representing the TID diff --git a/src/lib/position/pileup_position.rs b/src/lib/position/pileup_position.rs index 742a7cb..ca59710 100644 --- a/src/lib/position/pileup_position.rs +++ b/src/lib/position/pileup_position.rs @@ -107,7 +107,8 @@ impl PileupPosition { // Check if we are checking the base quality score // && Check if the base quality score is greater or equal to than the cutoff if let Some(base_qual_filter) = base_filter - && (record.seq().is_empty() || record.qual()[alignment.qpos().unwrap()] < base_qual_filter) + && (record.seq().is_empty() + || record.qual()[alignment.qpos().unwrap()] < base_qual_filter) { self.n += 1 } else if let Some(b) = recommended_base { @@ -222,7 +223,7 @@ impl PileupPosition { }) .sorted_by(|a, b| Ord::cmp(a.1.qname(), b.1.qname())) // TODO: I'm not sure there is a good way to remove this allocation - .group_by(|a| a.1.qname().to_owned()); + .chunk_by(|a| a.1.qname().to_owned()); for (_qname, reads) in grouped_by_qname.into_iter() { let mut total_reads = 0; // count how many reads there were @@ -547,11 +548,8 @@ mod tests { .unwrap(); // Read with empty SEQ at positions 10-35 - should count toward depth and N count - let empty_seq_record = Record::from_sam( - &view, - b"EMPTY_SEQ\t0\tchr1\t10\t40\t25M\t*\t0\t0\t*\t*", - ) - .unwrap(); + let empty_seq_record = + Record::from_sam(&view, b"EMPTY_SEQ\t0\tchr1\t10\t40\t25M\t*\t0\t0\t*\t*").unwrap(); // Another normal read with 'G' bases at positions 15-40 let normal_record2 = Record::from_sam( @@ -584,10 +582,14 @@ mod tests { for pileup_result in pileup_iter { let pileup = pileup_result.unwrap(); if pileup.pos() == 14 { - let position = PileupPosition::from_pileup(pileup, &header_view, &read_filter, None); + let position = + PileupPosition::from_pileup(pileup, &header_view, &read_filter, None); // Depth should include all 3 reads - assert_eq!(position.depth, 3, "Depth should be 3 (NORMAL + EMPTY_SEQ + NORMAL2)"); + assert_eq!( + position.depth, 3, + "Depth should be 3 (NORMAL + EMPTY_SEQ + NORMAL2)" + ); // 'A' count from NORMAL read assert_eq!(position.a, 1, "Should have 1 A from NORMAL read"); @@ -629,11 +631,8 @@ mod tests { .unwrap(); // Read with empty SEQ - let empty_seq_record = Record::from_sam( - &view, - b"EMPTY_SEQ\t0\tchr1\t1\t40\t25M\t*\t0\t0\t*\t*", - ) - .unwrap(); + let empty_seq_record = + Record::from_sam(&view, b"EMPTY_SEQ\t0\tchr1\t1\t40\t25M\t*\t0\t0\t*\t*").unwrap(); // Write BAM file let mut writer = Writer::from_path(&bam_path, &header, bam::Format::Bam).unwrap(); @@ -664,7 +663,10 @@ mod tests { assert_eq!(position.depth, 2, "Depth should be 2"); // 'A' from NORMAL (quality 'I' = 40, passes filter) - assert_eq!(position.a, 1, "Should have 1 A from high-quality NORMAL read"); + assert_eq!( + position.a, 1, + "Should have 1 A from high-quality NORMAL read" + ); // Empty SEQ read should be counted as N (regardless of base qual filter) assert_eq!(position.n, 1, "Empty SEQ read should be counted as N"); @@ -699,11 +701,8 @@ mod tests { .unwrap(); // Read with empty SEQ - let empty_seq_record = Record::from_sam( - &view, - b"EMPTY_SEQ\t0\tchr1\t1\t40\t25M\t*\t0\t0\t*\t*", - ) - .unwrap(); + let empty_seq_record = + Record::from_sam(&view, b"EMPTY_SEQ\t0\tchr1\t1\t40\t25M\t*\t0\t0\t*\t*").unwrap(); // Write BAM file let mut writer = Writer::from_path(&bam_path, &header, bam::Format::Bam).unwrap(); diff --git a/src/lib/read_filter.rs b/src/lib/read_filter.rs index bca1890..c0a6a82 100644 --- a/src/lib/read_filter.rs +++ b/src/lib/read_filter.rs @@ -31,8 +31,8 @@ impl ReadFilter for DefaultReadFilter { #[inline(always)] fn filter_read(&self, read: &Record, _alignment: Option<&Alignment>) -> bool { let flags = read.flags(); - (!flags) & &self.include_flags == 0 - && flags & &self.exclude_flags == 0 - && &read.mapq() >= &self.min_mapq + (!flags) & self.include_flags == 0 + && flags & self.exclude_flags == 0 + && read.mapq() >= self.min_mapq } } diff --git a/src/lib/utils.rs b/src/lib/utils.rs index fbb5f87..58d2f4b 100644 --- a/src/lib/utils.rs +++ b/src/lib/utils.rs @@ -29,10 +29,10 @@ pub fn set_rayon_global_pools_size(size: usize) -> Result<()> { /// Check if err is a broken pipe. #[inline] pub fn is_broken_pipe(err: &Error) -> bool { - if let Some(io_err) = err.root_cause().downcast_ref::() { - if io_err.kind() == io::ErrorKind::BrokenPipe { - return true; - } + if let Some(io_err) = err.root_cause().downcast_ref::() + && io_err.kind() == io::ErrorKind::BrokenPipe + { + return true; } false }