Starfish does not currently over-sample images, which means that there are edge effects that are introduced by filtering and segmentation that may not be recoverable unless users provide images with adequate overlaps.
This is not currently communicated well in our documentation. We should decide whether we want to support non-overlapping data by loading fields of view that are adjacent to the target field of view, or to request users submit fields of view that have overlaps, and then to support that in all algorithms.
In the short term, the existing limitations of starfish should be clearly documented.
Ref slack conversation: https://spacetx.slack.com/archives/CAR91U354/p1553107970076100