You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Copy file name to clipboardExpand all lines: src/content/viral-phylogenetics/steps/Step3.md
+3-8Lines changed: 3 additions & 8 deletions
Display the source diff
Display the rich diff
Original file line number
Diff line number
Diff line change
@@ -8,26 +8,21 @@ Now that we have run MSA on our dataset, we can perform Phylogenetic Inference:
8
8
9
9
We will use [FastTree](https://morgannprice.github.io/fasttree/) to generate an unrooted phylogenetic tree to assess relative ancestral relationships between our samples.
10
10
11
-
1. Try <Executecommand="FastTree -help"inline /> to take a look at the usage instructions.
11
+
1. Try <Executecommand="FastTree"inline /> to take a look at the usage instructions.
-`-nt` specifies that our alignment is of **n**ucleo**t**ides and not amino acids
20
20
21
-
-`sarscov2_sequences.msa.fas > sarscov2_sequences.unrooted_tree.nwk` tells FastTree to take in our multiple sequence alignment file (from Step 1) as input and to output the unrooted phylogenetic tree to a file called `sarscov2_sequences.unrooted_tree.nwk` in the same directory. A `.nwk` file is in Newick format, which is often used to represent phylogenetic trees. It is a text-based way to represent the tree structure. You can read more about Newick format [here](https://en.wikipedia.org/wiki/Newick_format).
22
-
23
-
**Optional Options:**
24
-
25
-
Note: The following options may be included in the command to improve accuracy at the cost of increased runtime.
26
-
27
21
-`-gtr` implements use of a [Generalized Time-Reversible](https://en.wikipedia.org/wiki/Substitution_model#Generalised_time_reversible) (GTR) model of evolution for our tree. FastTree can be run with either the [Jukes-Cantor](https://en.wikipedia.org/wiki/Models_of_DNA_evolution#JC69_model_(Jukes_and_Cantor_1969)) or GTR model.
28
22
29
23
-`-gamma` allows for rescaling of the branch lengths and computation of a Gamma2-based likelihood
30
24
25
+
-`sarscov2_sequences.msa.fas > sarscov2_sequences.unrooted_tree.nwk` tells FastTree to take in our multiple sequence alignment file (from Step 1) as input and to output the unrooted phylogenetic tree to a file called `sarscov2_sequences.unrooted_tree.nwk` in the same directory. A `.nwk` file is in Newick format, which is often used to represent phylogenetic trees. It is a text-based way to represent the tree structure. You can read more about Newick format [here](https://en.wikipedia.org/wiki/Newick_format).
31
26
32
27
3. Use `head` to view the first 10 lines of the tree file:
0 commit comments