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Update README.md
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README.md

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@@ -4,8 +4,8 @@ a computational pipeline for typing the Borrelia OspA protein
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## Installation
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Clone the repository and enter the directory:
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git clone https://github.com/Pfizer-rd/Lyme-In-Silico-Typing-Tool.git
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cd Lyme-In-Silico-Typing-Tool
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git clone https://github.com/Pfizer-opensource/LISTT.git
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cd LISTT
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Build and activate the environment:
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LISTT takes an assembled _Borrelia_ genome or Illumina NGS reads of the _ospA_ gene as input:
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python ospa_typing.py -r1 [read1 fastq] -r2 [read2 fastq] -p [path to fastq files] -m r
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python ospa_typing.py -r1 [read1 fastq] -r2 [read2 fastq] -m r
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python ospa_typing.py -g [genome fasta] -p [path to genome] -m a
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python ospa_typing.py -g [genome fasta] -m a
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Users may also specify the input file(s) location [-p] if an absolute path is not provided.
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Analysis will fail if there is insufficient coverage of _ospA_ or if _ospA_ is truncated in the genome assembly.
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