diff --git a/src/main/java/com/glencoesoftware/omero/zarr/ZarrStore.java b/src/main/java/com/glencoesoftware/omero/zarr/ZarrStore.java
index 1dec9e9a..0a149c5f 100644
--- a/src/main/java/com/glencoesoftware/omero/zarr/ZarrStore.java
+++ b/src/main/java/com/glencoesoftware/omero/zarr/ZarrStore.java
@@ -108,12 +108,12 @@ public class ZarrStore {
      */
     public ZarrStore(final String orgPath) throws URISyntaxException, IllegalArgumentException,
         IOException, ZarrException {
-        this.path = orgPath;
-        int zarrIndex = orgPath.lastIndexOf(".zarr");
+        this.path = normalizePath(orgPath);
+        int zarrIndex = path.lastIndexOf(".zarr");
         if (zarrIndex < 0) {
             throw new IllegalArgumentException("Path is not a .zarr");
         }
-        String pathToZarr = orgPath.substring(0, zarrIndex + 5);
+        String pathToZarr = path.substring(0, zarrIndex + 5);
         if (!path.contains("://") || path.startsWith("file")) {
             int sep = path.lastIndexOf(File.separator);
             String storePath = path.substring(0, sep);
@@ -227,6 +227,23 @@ public static String[] splitOnQuery(String uri) {
         return pathAndQuery;
     }
 
+    /**
+     * Removes trailing slashes from the path component of the given URI while preserving any
+     * query string. Trailing slashes otherwise produce empty path segments when the URI is
+     * split, which leads to malformed S3 keys such as {@code path//zarr.json}.
+     *
+     * @param orgPath the original URI, possibly ending in one or more slashes
+     * @return the URI with trailing slashes removed from the path component
+     */
+    static String normalizePath(String orgPath) {
+        String[] pathAndQuery = splitOnQuery(orgPath);
+        String normalized = pathAndQuery[0];
+        while (normalized.endsWith("/")) {
+            normalized = normalized.substring(0, normalized.length() - 1);
+        }
+        return normalized + (pathAndQuery[1] != null ? pathAndQuery[1] : "");
+    }
+
     private void assertNoAwsSystemEnvCredentials() {
         // If AWS Environment or System Properties are set, throw an exception
         // so users will know they are not supported
diff --git a/src/test/java/com/glencoesoftware/omero/zarr/ZarrStoreTest.java b/src/test/java/com/glencoesoftware/omero/zarr/ZarrStoreTest.java
index 5ea7ad97..015da54a 100644
--- a/src/test/java/com/glencoesoftware/omero/zarr/ZarrStoreTest.java
+++ b/src/test/java/com/glencoesoftware/omero/zarr/ZarrStoreTest.java
@@ -66,4 +66,37 @@ public void testRemoveNothing() {
         Assert.assertEquals("/my/test/path.zarr", pathAndQuery[0]);
         Assert.assertNull(pathAndQuery[1]);
     }
+
+    @Test
+    public void testNormalizePathStripsTrailingSlashes() {
+        Assert.assertEquals(
+                "s3://host/bucket/image.zarr/0?anonymous=true",
+                ZarrStore.normalizePath(
+                        "s3://host/bucket/image.zarr/0/?anonymous=true"));
+
+        Assert.assertEquals(
+                "s3://host/bucket/image.zarr/0?anonymous=true",
+                ZarrStore.normalizePath(
+                        "s3://host/bucket/image.zarr/0///?anonymous=true"));
+
+        Assert.assertEquals(
+                "s3://host/bucket/image.zarr/0",
+                ZarrStore.normalizePath(
+                        "s3://host/bucket/image.zarr/0/"));
+
+        Assert.assertEquals(
+                "https://host/image.zarr",
+                ZarrStore.normalizePath(
+                        "https://host/image.zarr/"));
+
+        Assert.assertEquals(
+                "s3://host/bucket/image.zarr",
+                ZarrStore.normalizePath(
+                        "s3://host/bucket/image.zarr"));
+
+        Assert.assertEquals(
+                "/my/test/path.zarr?anonymous=true",
+                ZarrStore.normalizePath(
+                        "/my/test/path.zarr?anonymous=true"));
+    }
 }