I run the pychopper with DCS108 data. I use DCS primers as provided by Nanopore: >VNP ACTTGCCTGTCGCTCTATCTTCTTTTTTTTT >SSP TTTCTGTTGGTGCTGATATTGCTGCCATTACGGCCGGG Th sequences are taken from https://community.nanoporetech.com/attachments/1459/download I follow your example for custom primers usage: cdna_classifier.py -m edlib -b custom_pimers.fas -c primer_config.txt input.fq full_length_output.fq However, the pychopper output still includes line saying "Using kit: PCS109" Why I still have this line ? May I be sure that the correct DCS108 primers have been actually used ?
I run the pychopper with DCS108 data.
I use DCS primers as provided by Nanopore:
Th sequences are taken from
https://community.nanoporetech.com/attachments/1459/download
I follow your example for custom primers usage:
cdna_classifier.py -m edlib -b custom_pimers.fas -c primer_config.txt input.fq full_length_output.fq
However, the pychopper output still includes line saying "Using kit: PCS109"
Why I still have this line ?
May I be sure that the correct DCS108 primers have been actually used ?