diff --git a/.github/workflows/ci.yaml b/.github/workflows/ci.yaml
index 0df211f0..183196bb 100644
--- a/.github/workflows/ci.yaml
+++ b/.github/workflows/ci.yaml
@@ -7,7 +7,7 @@ on:
     types: [run-all-tool-tests-command]
 env:
   GALAXY_FORK: galaxyproject
-  GALAXY_BRANCH: release_25.0
+  GALAXY_BRANCH: release_25.1
   MAX_CHUNKS: 40
 jobs:
   setup:
diff --git a/.github/workflows/pr.yaml b/.github/workflows/pr.yaml
index 935f9886..31e5719c 100644
--- a/.github/workflows/pr.yaml
+++ b/.github/workflows/pr.yaml
@@ -17,7 +17,7 @@ on:
       - '*'
 env:
   GALAXY_FORK: galaxyproject
-  GALAXY_BRANCH: release_25.0
+  GALAXY_BRANCH: release_25.1
   MAX_CHUNKS: 4
   MAX_FILE_SIZE: 1M
 concurrency: