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![diffUSE Project logo](/assets/images/diffuse_logo_banner.jpg){:style="max-height:300px; display: block; margin-left: auto; margin-right: auto;"}
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## Unlocking Protein Dynamics Through X-Ray Crystallography
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Structural biology has long been a key enabler for drug discovery, and for understanding protein function. As a field, structural biology has come a long way towards our understanding of static protein structures. But, proteins are dynamic and their functions are critically linked to fluctuations in state and motion. Our ability to measure these protein motions experimentally has been limited. To unlock the next frontier of advancements, we need more new method advancements and more experimental data to measure the dynamics of proteins.
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Structural biology has long been a key enabler for drug discovery, and for understanding protein function. As a field, structural biology has come a long way towards our understanding of static protein structures. But, proteins are dynamic and their functions are critically linked to fluctuations in state and motion. Our ability to measure these protein motions experimentally has been limited. To unlock the next frontier of advancements, we need more new method advancements, more experimental data, and ways to model and encode the dynamics of proteins.
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<!-- ![diffuse scattering slice](/assets/images/20250624_mapslice.png){:style="max-height:220px; display: inline; margin-left: auto; margin-right: auto;"} -->
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![diffuse scattering signals](/assets/images/diffuse_signals.png){:style="max-height:300px; display: block; margin-left: auto; margin-right: auto;"}
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The Diffuse Project is dedicated to advancing our understanding of protein motion through the use of diffuse scattering: a signal measured by X-ray crystallography. Most diffuse scattering data is currently ignored, but it could unlock our ability to measure protein dynamics.
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The Diffuse Project is dedicated to advancing our understanding of protein motion through the use of diffuse scattering: a signal measured by X-ray crystallography, which is currently ignored, but could unlock our ability to measure protein dynamics. We are also focused on building the infrastructure for learning and representing the rich information from this data.
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The Diffuse Project was co-developed with funders and a team of software developers, beamline scientists, structural biologists (experimental and computational), and modelers. The team includes both dedicated staff and institutional researchers to enable fast-paced infrastructure-building and specialized basic research. We will tackle the problem at a systems level by jointly iterating on data collection, sample preparation, hardware, software development, data processing and modeling methods.
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The Diffuse Project was co-developed with funders and a team of software developers, beamline scientists, structural biologists (experimental and computational), and modelers. The team includes both dedicated staff and institutional researchers to enable fast-paced infrastructure-building and specialized basic research. We will tackle the problem at a systems level by jointly iterating on data collection, sample preparation, hardware, software development, modeling algorithm development, and encoding strategies.
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![MD simulated diffuse scattering](/assets/images/20250805_Mac1_diffuse_crop.png){:style="max-height:300px; display: block; margin-left: auto; margin-right: auto;"}
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#### Publishing
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We want our science to be rigorous. We also want our tools to be useful adoption of our methods across the structural biology community will drive the impact of our work across basic and translational science. This means opening up more of our process as quickly as possible.
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We want our science to be rigorous. We also want our tools to be useful, as adoption of our methods across the structural biology community will drive the impact of our work across basic and translational science. This means opening up more of our process as quickly as possible.
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Therefore, we’re an open science project experimenting with novel [publishing practices](https://zenodo.org/records/15548989) outside of traditional journals, in accordance with Astera’s publication policy. The Diffuse Project is committed to transparency, collaboration, and scientific reproducibility. We’re exploring tooling, governance, and participation models to make our work globally accessible across sectors.
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