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README.md

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# proteinsearch
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An automated web search for human antibodies labelling neurons and glia in the brain. Takes the direct output of Seurat's "FindMarkers()" and returns a data frame of antibodies based on information from the human protein atlas.
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# proteinsearch.py
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An automated web search for human antibodies labelling neurons and glia in the brain.
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This code takes the direct output of Seurat's `FindMarkers()` and returns a data frame of antibodies based on information from the human protein atlas.
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## Instructions:
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1. Save a `.csv` of the marker genes for your single cell RNA sequencing data: `write.csv(FindMarkers(data), markers.csv)`
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2. In your terminal, ensure that Selenium web driver is installed: `pip install selenium`
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3. Download Chrome driver [here](https://chromedriver.chromium.org/downloads).
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4. In the terminal, navigate to the directory holding `proteinsearch.py`.
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5. In the terminal, type: `python proteinsearch.py`
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6. Enter the absolute path of your marker gene csv.
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7. Enter the name of of the output file (be sure to include .csv at the end).
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8. Chrome driver will open and begin the search!

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