diff --git a/python/tvm_ffi/core.pyi b/python/tvm_ffi/core.pyi
index c7b35b76..deb6c9f5 100644
--- a/python/tvm_ffi/core.pyi
+++ b/python/tvm_ffi/core.pyi
@@ -190,6 +190,7 @@ class DLTensorTestWrapper:
 def _dltensor_test_wrapper_c_dlpack_from_pyobject_as_intptr() -> int: ...
 
 class Function(Object):
+    def __init__(self, func: Callable[..., Any] | None = None) -> None: ...
     @property
     def release_gil(self) -> bool: ...
     @release_gil.setter
diff --git a/python/tvm_ffi/cython/function.pxi b/python/tvm_ffi/cython/function.pxi
index f66d7dfb..5e6257dc 100644
--- a/python/tvm_ffi/cython/function.pxi
+++ b/python/tvm_ffi/cython/function.pxi
@@ -889,6 +889,32 @@ cdef class Function(CObject):
     def __cinit__(self) -> None:
         self.c_release_gil = _RELEASE_GIL_BY_DEFAULT
 
+    def __init__(self, func: Optional[Callable[..., Any]] = None) -> None:
+        """Initialize a Function from a Python callable.
+
+        This constructor allows creating a `tvm_ffi.Function` directly
+        from a Python function or another `tvm_ffi.Function` instance.
+
+        Parameters
+        ----------
+        func : Optional[Callable[..., Any]]
+            The Python callable to wrap. When ``None`` (the default),
+            the object is left in its default state with a null handle.
+        """
+        if func is None:
+            return
+        cdef TVMFFIObjectHandle chandle = NULL
+        if isinstance(func, Function):
+            chandle = (<CObject>func).chandle
+            if chandle == NULL:
+                raise ValueError("Cannot initialize from a moved-from Function object.")
+            TVMFFIObjectIncRef(chandle)
+        elif callable(func):
+            _convert_to_ffi_func_handle(func, &chandle)
+        else:
+            raise TypeError(f"func must be callable, got {type(func)}")
+        self.chandle = chandle
+
     property release_gil:
         """Whether calls release the Python GIL while executing."""
 
diff --git a/tests/python/test_function.py b/tests/python/test_function.py
index 686fc08e..4bd7b8a8 100644
--- a/tests/python/test_function.py
+++ b/tests/python/test_function.py
@@ -153,6 +153,38 @@ def fapply(f: Any, *args: Any) -> Any:
     assert fapply(add, 1, 3.3) == 4.3
 
 
+def test_pyfunc_init() -> None:
+    def add(a: int, b: int) -> int:
+        return a + b
+
+    # Test creating from a Python callable
+    fadd = tvm_ffi.Function(add)
+    assert isinstance(fadd, tvm_ffi.Function)
+    assert fadd(1, 2) == 3
+
+    # Test creating from an existing tvm_ffi.Function
+    fadd2 = tvm_ffi.Function(fadd)
+    assert isinstance(fadd2, tvm_ffi.Function)
+    assert fadd2(3, 4) == 7
+    assert fadd.same_as(fadd2)
+
+    # Test creating from a moved-from function raises ValueError
+    f_source = tvm_ffi.Function(add)
+    f_dest = tvm_ffi.Function.__new__(tvm_ffi.Function)
+    f_dest.__move_handle_from__(f_source)
+    with pytest.raises(ValueError, match="Cannot initialize from a moved-from Function object"):
+        tvm_ffi.Function(f_source)
+
+    # Test creating without arguments (backward compat: null handle)
+    f_empty = tvm_ffi.Function()
+    assert isinstance(f_empty, tvm_ffi.Function)
+    assert f_empty.__chandle__() == 0
+
+    # Test creating from a non-callable raises TypeError
+    with pytest.raises(TypeError):
+        tvm_ffi.Function(123)  # ty: ignore[invalid-argument-type]
+
+
 def test_global_func() -> None:
     @tvm_ffi.register_global_func("mytest.echo")
     def echo(x: Any) -> Any: