How to co-assembly more than 9 samples?

[zhangtianyuan666]

Description of bug

run 9 samples = ok
run 10 samples = error

metaspades.py --only-assembler -k 21,33,55,77 -o cospades -t 200 -m 700 --pe1-1 CK-1.noHost.R1.fastq --pe1-2 CK-1.noHost.R2.fastq --pe2-1 CK-2.noHost.R1.fastq --pe2-2 CK-2.noHost.R2.fastq --pe3-1 CK-3.noHost.R1.fastq --pe3-2 CK-3.noHost.R2.fastq --pe4-1 CK-4.noHost.R1.fastq --pe4-2 CK-4.noHost.R2.fastq --pe5-1 CK-5.noHost.R1.fastq --pe5-2 CK-5.noHost.R2.fastq --pe6-1 SC-1.noHost.R1.fastq --pe6-2 SC-1.noHost.R2.fastq --pe7-1 SC-2.noHost.R1.fastq --pe7-2 SC-2.noHost.R2.fastq --pe8-1 SC-3.noHost.R1.fastq --pe8-2 SC-3.noHost.R2.fastq --pe9-1 SC-4.noHost.R1.fastq --pe9-2 SC-4.noHost.R2.fastq --pe10-1 SC-5.noHost.R1.fastq --pe10-2 SC-5.noHost.R2.fastq

== Warning == output dir is not empty! Please, clean output directory before run.

SPAdes genome assembler v4.1.0 [metaSPAdes mode]

Usage: spades.py [options] -o <output_dir>
spades.py: error: Please specify option (e.g. -1, -2, -s, etc)) for the following paths: --pe10-1, SC-5.noHost.R1.fastq, --pe10-2, SC-5.noHost.R2.fastq

spades.log

Not avaliable

params.txt

Not avaliable

SPAdes version

4.10

Operating System

Ubuntu

Python Version

3.8.4

Method of SPAdes installation

conda

No errors reported in spades.log

• Yes

[asl]

Do you really need more than 9 separate libraries? Are all these files have different characteristics? If yes, consider using YAML dataset file description: https://ablab.github.io/spades/running.html#specifying-multiple-libraries-with-yaml-data-set-file