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It might be worth considering including some images for the Golgi in brefeldin-treated cells as well as for microtubules in taxol-treated cells. It is clear that there is a population for the treated cells that is missing from the untreated (between -2 and -4 on the horizontal axis), as well as some cells that fall into the bulk of the distribution for untreated cells. It would be helpful to have at least one image for each. This is a low dose of brefeldin as such things go, so it is to be expected that some cells are not responding.
Is there a clear explanation for why the contingent latent spaces for tight junctions and for the Golgi are so narrowly distributed compared to the microtubules? I expect this has something to do with the actual intrinsic cell-to-cell variation for those structures. It should be at least noted.
It seems inappropriate to “interpret” the blue and pink bars for tight junctions in part C as revealing a role for microtubules in tight junction formation (lines 268-269). The actual difference between this and the brefeldin result is miniscule, regardless of which bar gets the star, and the magnitude (<0.5 standard deviations) is not sufficient to draw a biological conclusion without any further support.
TODO
- manuscript text